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Structured Review

Sangon Biotech plasmids
Plasmids, supplied by Sangon Biotech, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/plasmids/plasmids/pmc12993211-57-2-9
Average 86 stars, based on 1 article reviews
plasmids - by Bioz Stars, 2026-09
86/100 stars

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Related Articles

Recombinant:

Article Title: Fusion expression of carbohydrate-binding module and linker peptide improves the product specificity of chitosanase.
Article Snippet: The efficacy of chitooligosaccharides (COS) is closely related to their degree of polymerization (DP), with higher–DP COS offering significant bioactivities and stability advantages.. The objective of this study is to enhance the product specificity of chitosanase for producing higher-DP COS.. In this study, we proposed a novel strategy to enhance the affinity and activity of the chitosanase Csn75 through the fusion of a carbohydratebinding module CBM32 (specifically its DD1 subdomain) using rigid or flexible linkers of varying lengths.

Article Title: Structure-coevolution dual-guided engineering of C-glycosyltransferase enables high-level biosynthesis of vitexin in Yarrowia lipolytica
Article Snippet: Mutations were introduced using the Mut Express II Fast Mutagenesis Kit V2 (Vazyme, China). .. The recombinant plasmids were transformed into E. coli BL21(DE3) (Sangon Biotech, China) for heterologous protein expression. ..

Article Title: Identification and characteristics of a novel acquired aminoglycoside phosphotransferase, APH(3′)-IVb, from Riemerella anatipestifer
Article Snippet: The aph(3 ′ )-IVb gene was cloned into the pET32a and pLMF03 vectors using a seamless cloning strategy (Sangon Biotech, Shanghai, China). .. The resulting recombinant plasmids, pET32a- aph(3 ′ )-IVb and pLMF03- aph(3 ′ )-IVb , were verified by Sanger sequencing (Sangon Biotech) and subsequently transformed into E. coli BL21(DE3) and R. anatipestifer ATCC 11845, respectively. ..

Article Title: Engineering a Virus-Derived X Family DNA Polymerase FvPolX for de novo DNA Synthesis.
Article Snippet: Vector pETDuet-1 was digested with restriction enzymes Bam HI and Not I. .. The recombinant plasmids were first transformed into cloning strains, and single colonies were picked and sent to Sangon Biotech (Qingdao, China) for DNA sequencing. ..

Synthesized:

Article Title: Fusion expression of carbohydrate-binding module and linker peptide improves the product specificity of chitosanase.
Article Snippet: The efficacy of chitooligosaccharides (COS) is closely related to their degree of polymerization (DP), with higher–DP COS offering significant bioactivities and stability advantages.. The objective of this study is to enhance the product specificity of chitosanase for producing higher-DP COS.. In this study, we proposed a novel strategy to enhance the affinity and activity of the chitosanase Csn75 through the fusion of a carbohydratebinding module CBM32 (specifically its DD1 subdomain) using rigid or flexible linkers of varying lengths.

Sequencing:

Article Title: Modular reconstruction and multilevel regulation of Saccharomyces cerevisiae to drive the efficient synthesis of asiatic acid
Article Snippet: .. All primers and plasmids passed Sanger sequencing by Sangon Biotechnology Co., Ltd. .. The single gene editing system of S. cerevisiae utilizes the CRISPR-Cas9 system, and the prediction and design of sgRNAs is performed using the Benchling ( https://benchling.com ) website.

Article Title: Identification and characteristics of a novel acquired aminoglycoside phosphotransferase, APH(3′)-IVb, from Riemerella anatipestifer
Article Snippet: The aph(3 ′ )-IVb gene was cloned into the pET32a and pLMF03 vectors using a seamless cloning strategy (Sangon Biotech, Shanghai, China). .. The resulting recombinant plasmids, pET32a- aph(3 ′ )-IVb and pLMF03- aph(3 ′ )-IVb , were verified by Sanger sequencing (Sangon Biotech) and subsequently transformed into E. coli BL21(DE3) and R. anatipestifer ATCC 11845, respectively. ..

DNA Purification:

Article Title: Microfluidic-free droplet-based CRISPR/Cas12a assay for semi-digital detection of DNA methylation
Article Snippet: The TwistAmp Basic RPA kit was purchased from TwistDx Limited (Maidenhead, U.K.). .. DNA purification kits, RNase/DNase-free water, AGE reagents and plasmids carrying human FOXD3, and ssDNA-FQ reporters were obtained from Sangon Biotech (Shanghai, China). .. Bovine serum albumin (BSA), magnesium acetate, and NaCl were purchased from Macklin (Shanghai, China).

FLAG-tag:

Article Title: Low-density lipoprotein receptor–related protein 1 mediates α-synuclein transmission from the striatum to the substantia nigra in animal models of Parkinson’s disease
Article Snippet: .. Plasmids encoding a Flag tag (Flag), Flag-tagged full-length human α-syn (Flag- SNCA ), Flag-tagged α-syn N-terminus (Flag-N- SNCA ), and Flag-tagged α-syn missing the N-terminus (Flag-ΔN- SNCA ), as well as a NC plasmid (pcDNA3.1+ plasmid), were purchased from Sangon Biotech (Shanghai) Co., Ltd. (Shanghai, China). ..

Plasmid Preparation:

Article Title: Low-density lipoprotein receptor–related protein 1 mediates α-synuclein transmission from the striatum to the substantia nigra in animal models of Parkinson’s disease
Article Snippet: .. Plasmids encoding a Flag tag (Flag), Flag-tagged full-length human α-syn (Flag- SNCA ), Flag-tagged α-syn N-terminus (Flag-N- SNCA ), and Flag-tagged α-syn missing the N-terminus (Flag-ΔN- SNCA ), as well as a NC plasmid (pcDNA3.1+ plasmid), were purchased from Sangon Biotech (Shanghai) Co., Ltd. (Shanghai, China). ..

Transformation Assay:

Article Title: Structure-coevolution dual-guided engineering of C-glycosyltransferase enables high-level biosynthesis of vitexin in Yarrowia lipolytica
Article Snippet: Mutations were introduced using the Mut Express II Fast Mutagenesis Kit V2 (Vazyme, China). .. The recombinant plasmids were transformed into E. coli BL21(DE3) (Sangon Biotech, China) for heterologous protein expression. ..

Article Title: Identification and characteristics of a novel acquired aminoglycoside phosphotransferase, APH(3′)-IVb, from Riemerella anatipestifer
Article Snippet: The aph(3 ′ )-IVb gene was cloned into the pET32a and pLMF03 vectors using a seamless cloning strategy (Sangon Biotech, Shanghai, China). .. The resulting recombinant plasmids, pET32a- aph(3 ′ )-IVb and pLMF03- aph(3 ′ )-IVb , were verified by Sanger sequencing (Sangon Biotech) and subsequently transformed into E. coli BL21(DE3) and R. anatipestifer ATCC 11845, respectively. ..

Article Title: Engineering a Virus-Derived X Family DNA Polymerase FvPolX for de novo DNA Synthesis.
Article Snippet: Vector pETDuet-1 was digested with restriction enzymes Bam HI and Not I. .. The recombinant plasmids were first transformed into cloning strains, and single colonies were picked and sent to Sangon Biotech (Qingdao, China) for DNA sequencing. ..

Expressing:

Article Title: Structure-coevolution dual-guided engineering of C-glycosyltransferase enables high-level biosynthesis of vitexin in Yarrowia lipolytica
Article Snippet: Mutations were introduced using the Mut Express II Fast Mutagenesis Kit V2 (Vazyme, China). .. The recombinant plasmids were transformed into E. coli BL21(DE3) (Sangon Biotech, China) for heterologous protein expression. ..

Cloning:

Article Title: Engineering a Virus-Derived X Family DNA Polymerase FvPolX for de novo DNA Synthesis.
Article Snippet: Vector pETDuet-1 was digested with restriction enzymes Bam HI and Not I. .. The recombinant plasmids were first transformed into cloning strains, and single colonies were picked and sent to Sangon Biotech (Qingdao, China) for DNA sequencing. ..

DNA Sequencing:

Article Title: Engineering a Virus-Derived X Family DNA Polymerase FvPolX for de novo DNA Synthesis.
Article Snippet: Vector pETDuet-1 was digested with restriction enzymes Bam HI and Not I. .. The recombinant plasmids were first transformed into cloning strains, and single colonies were picked and sent to Sangon Biotech (Qingdao, China) for DNA sequencing. ..

Small Interfering RNA:

Article Title: Astaxanthin alleviates cerebral infarction by targeting MAPK14: insights from network pharmacology, Molecular Docking, and experimental validation
Article Snippet: .. The small interfering RNA (siRNA) used for constructing MAPK14 knockdown (si-MAPK14) or plasmids used for constructing MAPK14 overexpression (OE-MAPK14) were sourced from Sangon Biotech (Shanghai, China). ..

Knockdown:

Article Title: Astaxanthin alleviates cerebral infarction by targeting MAPK14: insights from network pharmacology, Molecular Docking, and experimental validation
Article Snippet: .. The small interfering RNA (siRNA) used for constructing MAPK14 knockdown (si-MAPK14) or plasmids used for constructing MAPK14 overexpression (OE-MAPK14) were sourced from Sangon Biotech (Shanghai, China). ..

Over Expression:

Article Title: Astaxanthin alleviates cerebral infarction by targeting MAPK14: insights from network pharmacology, Molecular Docking, and experimental validation
Article Snippet: .. The small interfering RNA (siRNA) used for constructing MAPK14 knockdown (si-MAPK14) or plasmids used for constructing MAPK14 overexpression (OE-MAPK14) were sourced from Sangon Biotech (Shanghai, China). ..



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